Gonadotrophin releasing hormone (GnRH) release is the primary regulator of the reproductive axis. A major regulator of GnRH secretion is kisspeptin, which does so integrating a wide array of information concerning the external and internal environment. Little is known about GnRH and kisspeptin in marsupials. We examined the distribution of GnRH and kisspeptin immunoreactivity in the brains of female adult tammars. The heads (n=14) were perfusion fixed (4% paraformaldehyde) and the brains dissected out and frozen. Immunohistochemistry for GnRH and kisspeptin was performed on 40µm cryostat sections, using fluorescently labelled secondary antibodies. GnRH cell bodies were localised primarily to the medial septum, especially immediately dorsal to the anterior commissure, with small numbers in the preoptic area and arcuate nucleus. This is further rostral and dorsal than that seen in eutherian mammals. Fibres projected in a remarkably ordered manner ventrally and then caudally to the median eminence, which contained a dense plexus of GnRH-containing terminals. These fibres ran in parallel as a tight group, with little in other parts of the hypothalamus. Unlike other species, where Kisspeptin neurons are found primarily in the arcuate nucleus, neurones were located in the dorsomedial hypothalamus, with few in the arcuate nucleus, and none in the anterior periventricular region. Kisspeptin fibres were scattered widely throughout the hypothalamus, with labelling in the medial basal hypothalamus, but few fibres were found in the median eminence. Pre-adsorption studies confirmed no cross reactivity between the kisspeptin antibody and gonadotrophin inhibitory hormone. These results indicate that GnRH and kisspeptin peptides similar to eutherian mammals are present in the wallaby brain and similar, but not identical, locations to those observed in other mammalian species. We saw little evidence of kisspeptin-GnRH interactions, which casts into doubt whether kisspeptin may be a major player in the regulation of GnRH secretion in this species.