PUMA regulates germ cell apoptosis and primordial follicle endowment in mice — ASN Events

PUMA regulates germ cell apoptosis and primordial follicle endowment in mice (#14)

Michele Myers 1 2 , Hamish Morgan 2 , Seng Liew 2 , Michele Cook 3 , Nadeen Zerafa 2 , Upi Thilini 2 , Mai Sarraj 1 2 , Clare Scott 3 , Andreas Strasser 3 , Jock Findlay 2 , Jeff Kerr 1 , Karla Hutt 1 2
  1. Monash University, Clayton, Australia
  2. Prince Henry's Institute, Clayton, VIC, Australia
  3. Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

During embryonic and early postnatal ovarian development, more than two-thirds of the newly made germ cells undergo apoptosis for unknown reasons and by poorly characterised mechanisms. We have previously shown that postnatal day (PN) 10 mice deficient in the proapoptotic BH3-only protein PUMA have an oversupply of primordial follicles when compared to wild type (WT) mice (Kerr et al. Mol Cell 2012; 48(3):343-52), suggesting that PUMA is required for germ cell death prior to the establishment of the primordial follicle reserve. To confirm a role for PUMA in regulating germ cell death and setting the size of the initial primordial follicle pool, germ cells and follicles were enumerated in WT and Puma-/- ovaries during embryonic and early postnatal development (n=6/age/genotype). We found that the number of germ cells was significantly greater in embryonic Puma-/- ovaries compared to WT ovaries as early as embryonic day (E)13.5 (WT 2850±354 vs Puma-/- 6408±738, p<0.05). Germ cell numbers were also elevated in Puma-/- ovaries at E15.5, though the difference to WT was not significant (WT 14587 ± 1280 vs Puma-/- 21059 ± 3440, p=0.45). Between E17.5 and PN10, there was a significant reduction in germ cell numbers in both WT and Puma-/- ovaries, suggesting PUMA is not essential for apoptosis during germ cell nest break down and primordial follicle assembly. However, germ cell numbers did remain consistently higher in Puma-/- ovaries compared to WT ovaries during this time (E17.5, WT 13981 ± 4052 vs Puma-/- 24984 ± 405; PN3, WT 7111 ± 978 vs Puma-/- 10923 ± 966; PN10 WT 4534±396 vs Puma-/- 8488±1233; p<0.05). Thus, the 2-fold increase in germ cell number in Puma-/- mice at E13.5 was maintained throughout ovarian development and resulted in a doubling of the number primordial follicles initially established in the ovary. Our data indicate that PUMA is a critical regulator of germ cell apoptosis during their migration or very soon after their arrival at the gonad, and that PUMA-mediated cell death limits the number of primordial follicles established in the initial ovarian reserve.